Enzymatic Plastic Degradation, Volume 648 in the Methods in Enzymology series, continues the legacy of this premier serial with chapters authored by leaders in the field. Chapters in this latest release include Evaluating plastic pollution and environmental degradation, Assessment methods for microplastic pollution in the oceans and fresh water, Exploring microbial consortia from various environments for plastic degradation, Characterization of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry, Synthesis of radioactive-labeled nanoplastics for assaying the environmental (microbial) PS degradation, Exploring metagenome for plastic degrading enzymes, Cutinases from thermophilic bacteria (actinomycetes): from identification to functional and structural characterization, and much more.
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Table of Contents
1. Evaluating plastic pollution and environmental degradation Pia Skoczinski 2. Assessment methods for microplastic pollution in the oceans and fresh water Chengjun Sun 3. Exploring microbial consortia from various environments for plastic degradation Basak ?zt?rk and Ingrid Eileen Meyer Cifuentes 4. Characterization of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry Dietmar Schlosser 5. Culturing various mealworms for the degradation of PS/PE and the corresponding analysis of gut microbiota Wei-Min Wu 6. Synthesis of radioactive-labeled nanoplastics for assaying the environmental (microbial) PS degradation Rong Ji 7. Exploring metagenome for plastic degrading enzymes Wolfgang Streit 8. Cutinases from thermophilic bacteria (actinomycetes): from identification to functional & structural characterization ??? ?? 9. Ideonella sakaiensis & PETase and MHETase: from identification of microbial PET degradation to enzyme characterization Kohei Oda 10. Design of novel PETase mutants by machine-learning/AI-based strategy Bian Wu 11. High throughput screening assays for PET/PU hydrolase activities Ren Wei 12. Methods for evaluating the biodegradability of PET Chen Song 13. Heterogeneous kinetic analysis of the enzymatic PET hydrolysis Thomas Maskow 14. Adhesion peptide promoted and selective degradation of mixed plastics for enabling recycling Shohana Subrin Islam 15. Adsorption study of plastic degrading enzymes and monomer conversion for synthesis of virgin polymers Georg M. Guebitz 16. Characterization and screening of microbial degradation of PU Luc Averous 17. Structural and functional characterization of the PET oligomer degrader (MHETase, Carboxylesterases, etc.) Gert Weber and Gottfried J. Palm 18. Structural and functional characterization of nylon hydrolases Seiji Negoro 19. Bioprocess engineering for PET recycling Alain Marty 20. Engineering enzymes for embedding in PLA mulching films for rapid environmental degradation Alain Marty 21. The use of degraded plastic monomers as substrates for microbial growth and production of biodegradable plastics (and their biodegradation) Tanja Narancic 22. Culturing and engineering bacterial strains for up-cycling of plastic degradation products Till Tiso 23. Screening and cultivating microbial strains able to grow on degradation intermediates from polyurethane Christian Eberlein 24. Engineering microalgae as a whole cell biocatalyst for PET degradation Daniel Moog 25. Yeast Cell Surface Display Bacterial PETase as a Reusable Biocatalyst for Degradation of Highly Crystallized Polyethylene Terephthalate Zefang Wang