Ion Channels, Part C, Volume 653 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Nonsense suppression in ion channels, Engineering Ion Channels Using Protein Trans-splicing, Probing Ion Channel Neighborhoods Using APEX, STX based probes for NaVs, ANAP: a versatile, fluorescent probe of ion channel gating and regulation, High Throughput Screens for Small Molecule Ion Channel Modulators, Using toxins to study ion channels, Re/de-constructing ubiquitin regulation of ion channels, Tethered Peptide Toxins for Ion Channels, Voltage-Sensing Phosphatase Molecular Engineering, and more.
Additional chapters cover Engineering excitable cells, Stretch and Poke Stimulation of Mechanically-Activated Ion Channels, Optical Control of STIM Channels, High Throughput Electrophysiological Evaluation of Mutant Ion Channels, Evaluating BEST1 Mutations in RPE Stem Cells, Long Read Transcript Profiling of Ion Channel Splice Variants, Permeation of Connexin Channels, Ratiometric pH indicator for melanosomes and lysosomes, and Ion channels in the epithelial cells of the choroid plexus.
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Table of Contents
PrefaceDaniel L. Minor Jr. and Henry M. Colecraft
Methods to engineer ion channels to probe different aspects of channel structure-function and regulation.
1: Selection and validation of orthogonal tRNA / synthetase pairs for the encoding of unnatural amino acids across kingdoms. Christopher Ahern
2: Ion channel engineering using protein trans-splicing. Stephan Alexander Pless
3: ANAP: a versatile, fluorescent probe of ion channel gating and regulation. Michael C. Puljung
4: Engineering voltage sensing phosphatase, VSP. Yasushi Okamura
5: Probing Ion Channel Neighborhoods Using APEX. Steven Marx
Methods to control and characterize ion channel function
6: Controlling ion channel trafficking by targeted ubiquination and deubiquitination. Henry M. Colecraft
7: Production of recombinant venom peptides as tools for ion channel research. Eitan Reuveny
8: Tethered Peptide Toxins for Ion Channels. Steven A. N. Goldstein
9: Stretch and poke stimulation for characterizing mechanically activated ion channels. J?rg Grandl
10: Optogenetic control of calcium influx in mammalian cells. YUBIN ZHOU
11: A method for assessing ionic and molecular permeation in connexin hemichannels. JORGE E. CONTRERAS
Methods to characterize ion channel function and expression in physiology and disease
12: Choroid plexus epithelial cells as a model to study nongenomic steroid signaling and its effect on ion channel function. Polina V. Lishko
13: Live-cell measurements of individual melanosome pH using the novel ratiometric indicator RpHiMEL. Elena Oancea
14: Long Read Transcript Profiling of Ion Channel Splice Isoforms. Liz Tunbridge
15: Evaluating BEST1 Mutations in Pluripotent Stem Cell-derived Retinal Pigment Epithelial Cells. Tingting Yang
16: High Throughput Electrophysiological Evaluation of Mutant Ion Channels. Alfred L. George Jr.
17: In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy
Nenad Bursac
