Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers.
With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research.
Table of Contents
Preface 1: Supercritical excitation and emission near surfaces 2: Adaptive optics for fluorescence microscopy of thick tissue 3: Rapid polarization of fluorescence measurements in ex-vivo tissue 4: Microscopy and nanoscale membrane organization 5: Imaging entire organs 6: RNA mimics of GFP 7: High content/throughput imaging and cytometry via fluorescence and surface enhanced Raman scattering (SERS) microscopy 8: Light sheet microscopy applications 9: Cryo-ET-fluorescence super-resolution 10: STED applications 11: Use of engineered nanoparticles based on fluorescence methods for live cell phenomena 12: Illumination pattern correction 13: Confocal stereology 14: Multi-photon microscopy applications in biology 15: TIRF 16: SIM 17: The role of data analysis algorithms in super-resolution localization microscopy
Anda Cornea Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, USA.
Anda Cornea, Ph.D. is the Director of the Imaging and Morphology Core at the Oregon National Primate Research Center where she routinely uses and teaches novel fluorescence techniques. She has co-authored more than 30 peer-reviewed publications, edited a Methods volume and contributed chapters in several technical books. Anda holds a Ph.D. in Biological Sciences from Southern Methodist University in Dallas, TX, an M.S in Biophysics and a B.S. in Physics from the University of Bucharest, Romania. She was a postdoctoral Associate at the Howard Hughes Medical Institute at Rockefeller University in New York, before joining the Primate Center.
P. Michael Conn Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center, TX, USA.
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public's awareness of diabetes. Conn's students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.