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Biocatalyst Immobilization. Foundations and Applications. Foundations and Frontiers in Enzymology

  • Book
  • November 2022
  • Elsevier Science and Technology
  • ID: 5597167

Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes.

Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.

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Table of Contents

1. Early supports and methods: the beginning and the key findings to progress 2. Selection and modification of enzymes prior to immobilization 3. Selection of supports for immobilization 4. Some challenges during immobilization 5. Post-immobilization treatments before applications 6. Measuring and reporting immobilization efficiency 7. Various approaches to immobilization 8. Immobilization for use in non-conventional media 9. Assaying biological activities of the immobilized enzymes 10. Support-free immobilization 11. Immobilization on nanomaterials and nano-structured supports 12. Hyphenated and non-hyphenated techniques in immobilized enzymes characterization 13. Some recent innovations related to enzyme immobilixation 14. Water-soluble supports, bioconjugates and smart immobilized enzymes 15. Oriented Immobilization and Immobilization in small surfaces 16. Affinity immobilization and affinity layers 17. New enzymatic reactor designs: from enzymatic batch to 3D microreactors and monoliths 18. Immobilization of Multi-Enzymes: Problems and Solutions 19. An introduction to enzymatic Biosensors 20. Whole-Cell Immobilization at 2020 21. Industrial Applications of immobilized enzymes: Food and beyond 22. Final Future perspectives in enzyme immobilization


Maria Lujan Ferreira Principal Investigator, CONICET (National Council of Scientific and Technical Research), PLAPIQUI.-UNS-CONICET, Bahia Blanca, Buenos Aires, Argentina. Dr. María Luján Ferreira is a Principles Investigator at CONICET (NATIONAL COUNCIL OF SCIENTIFIC AND TECHNICAL RESEARCH) in Buenos Aires, R. Argentina. Her research focuses on practical aspects of enzyme immobilization, including biocatalysis sampling in applied biotechnology. She has published 144 articles in peer reviewed, international journals, and has presented at over 80 conferences and forums worldwide. Dr. Ferreira has also directed three Assistant Investigators at CONICET.