Stem Cell Laboratory Techniques. A Guide for Researchers and Students

Table of Contents

1. Introduction: Stem Cell Definition, Types of Stem Cells, Use and Application of Stem Cell
2. Extraction of Stem Cells from Animal Models
3. Harvest and Culture of Stem Cells
4. Establishment of Amniotic fluid Stem Cell
5. Stem Cell Characterization
6. Good Recording and Quality Control
7. Differentiation Techniques for Confirmation Studies
8. Transcriptomic Analysis
9. Proliferation Study and Analysis
10. Single Cell Isolation Methods and their Impact on Stem Cell Study
11. Gene Editing Techniques for Stem Cell
12. Preparation of Cells for in vitro study
13. Stem Cell Lab Design and Equipment
14. Human and Animal Research Ethics
15. Organoids

Authors

Suresh Kumar Subbiah Centre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India. Dr. Suresh Kumar is Scientist-SG & Group Head for the Agriculture & Soils Department at Indian Institute of Remote Sensing (IIRS), Govt. of India, Indian Space Research Organization (ISRO). He has 27 years of experience in applications of geospatial technologies in natural resource management with specialization in soil resource management, land degradation, and watershed management. He has published more than 50 research papers and 7 book chapters. He has attended several International Conferences and National Seminar/ Symposium and delivered invited lead talk in the seminar/symposium. He has also carried out several research projects as PI / Co-I, such as: FAO-AEZ based Agricultural Land Use Planning; National Soil Carbon Pool Assessment, Soil Carbon Dynamic (SCD) Studies, Mountain Ecosystem Processes and Services: Studying impact of Soil Erosion and Nutrient Loss and its impact on Soil Quality; Digital soil mapping using environmental covariates for mountainous region. Akon Higuchi State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China; Department of Chemical and Materials Engineering, National Central University, Taoyuan, Taiwan;
Center for Emergent Matter Science, Riken, Wako, Saitama, Japan. Prof. Higuchi is a distinguished Professor in National Central University, a guest researcher in RIKEN (Japan) and National Research Center for Child Health and Development (Tokyo), and a visiting professor in King Saud University (Saudi Arabia) and Wenzhou Medical University (China). He received his Ph.D. in Tokyo Institute of Technology in 1985. He was a Professor in Seikei University (Tokyo) from 1993 to 2007. He received Sofue Memorial Award from Society of Fiber Science, Japan in 1994, Seikei Academic Award from Seikei Alumni Association in 2003, and Nanotechnology Outstanding Contribution Award, National Science Council, Taiwan in 2013, Future Tech Breakthrough Award in 2019, Ministry of Science and Technology, Taiwan. He also received Outstanding Scientific Award, Tissue Engineering and Regenerative Medicine International Society, Asia Pacific region (TERMIS-AP) in 2021. He is a fellow of Royal Society of Chemistry (RSC, England), a fellow of Taiwan Chemical Engineering Society, and a fellow of American Institute for Medical & Biological Engineering (AIMBE, USA). He is interested in the development of materials for stem cell culture and differentiation. He established purification method of hematopoietic stem cells and mesenchymal stem cells from umbilical cord blood and adipose tissue, respectively by filtration method through polymeric porous membranes. He is also developing biomaterials for stem cell culture and differentiation. He emphasized the important function of cell culture biomaterials, which guide differentiation fate of human pluripotent stem cells and mesenchymal stem cells into specific lineage of the cells. He developed ECM-derived peptide-immobilized hydrogels having adjustable elasticity for culture and differentiation of human pluripotent stem cells. He is now developing universal (hypoimmunogenic) human pluripotent stem cells on specific ECM-coated surface. He is an advisory board of Journal of Materials Chemistry B (RSC), an Editor of Open Physics, Editorial Board of Laboratory Investigation, Editorial Board of Tissue Engineering and Regenerative Medicine, and Associate Editor of IET Nanobiotechnology. Pooi Ling Mok Department of Biomedical Science
Universiti Putra Malaysia, Selangor, Malaysia. Dr. Mok received her PhD award from National University of Malaysia, Malaysia in 2012. She joined the Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia in 2013 and is currently an Associate Professor in the Department of Biomedical Science. She also has previously served as an Assistant Professor in Jouf University, Saudi Arabia from 2017 to 2021. She is also the founding Vice President of Malaysian Stem Cell for Research and Therapy (MSCRT) association. Dr. Mok has passion for researches involving stem cell engineering at the genetic, cell and tissue level involving human samples with aim to improve the quality of life in the patients. Most of her research works integrated many methods that include genetic modifications, use of biomaterials and tissue engineering for optimal result outcomes. Her current research focus is to find alternative treatment for visual impairment using stem cells. Other than that, she is also conducting research on cancer targeting by using CRISPR-Cas technology and introduction of chimeric antigen receptor into immune effector cells.