Advanced Methodologies to Study and Enhance Cellular Cytotoxicity Against Cancer, Volume 207 covers the latest techniques and strategies used to investigate and boost cytotoxic activity in cells targeting cancer. Chapters include An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocol, New protocol to measure mitochondria polarization/function at the immunological synapse, Analysis of CAR-T cell-mediated cytotoxicity in solid tumor models, Investigation of the in vitro lytic capacity of CAR-T by microscopyal, Antigen-specific T cell cytotoxicity toward target tumor cells, In vitro expansion of stem-like memory T cells via D-mannose supplementation, and much more.
Additional chapters explore High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells, Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activation, Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometry, Super-resolution imaging of the NK cell synapse on supported lipid bilayers using MINFLUX nanoscopy, ASSESSMENT OF NK CELL CYTOTOXICITY INDUCED BY IL-15 BASED IMMUNOTHERAPY AGAINST CANCER CELLS, Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometry, and much more.
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Table of Contents
1. An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocolEmmanuel int�grative des tumeurs et immunoth�rapie Donnadieu, Irena Rajnpreht and Lene Vimeux
2. new protocol to measure mitochondria polarization/function at the immunological synapse
Manuel Izquierdo, Javier Ruiz Navarro and Victor Calvo
3. Analysis of CAR-T cell-mediated cytotoxicity in solid tumor models
Teresa Lozano, Noa Beatriz Mart�n-C�freces, �lvaro G�mez-Mor�n and Celia Mart�n-Otal
4. Investigation of the in vitro lytic capacity of CAR-T by microscopy'
Noa Beatriz Mart�n-C�freces, Teresa Lozano, �lvaro G�mez-Mor�n and Celia Mart�n-Otal
5. Antigen-specific T cell cytotoxicity toward target tumor cells
Chong Sun and Helena Engel
6. In vitro expansion of stem-like memory T cells via D-mannose supplementation
Bo Huang, Guideng Li, Yajing Qiu and Cngcong Zhao
7. High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells.
H�l�ne Sophie DANIELS-TREFFANDIER, Loic Dupre, Beatriz CHAVES and Nicolas SOCQUET-JUGLARD
8. Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activation
Yu Cheng, Rui Gao, Wanxin Huang and Yehe Kang
9. Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometry
Massimo Vitale, Paola Orecchia, Guido Ferlazzo, Monica Parodi, Simonetta Astigiano, Valentina Delli Zotti, Filippo Piombo, Stefania Martini, Simona Pigozzi and Paolo Carrega
10. Super-resolution imaging of the NK cell synapse on supported lipid bilayers using MINFLUX nanoscopy.
Charles Daniel Murin, Emily Mace, Patrick Ross, Jessica Matthias, Daniel Leaman, Micahel Zwick and Scott Henderson
11. ASSESSMENT OF NK CELL CYTOTOXICITY INDUCED BY IL-15 BASED IMMUNOTHERAPY AGAINST CANCER CELLS
Carole Seguin-Devaux, Camille Rolin, Gilles Iserentant and Aubin Pitiot
12. Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometry
Sophie-Christin Linkenbach, Ayline K�bler and Tommaso Torcellan
13. Anti-tumor activity characterization of NK cell subsets by flow cytometry
Massimo Vitale, Carola Perrone, Paolo Canevali, Veronica Colamassaro, Elena Giusto, Silvia Tamburini, Gianluca Ubezio, Guido Ferlazzo and Raffaella Meazza
14. DNA Origami-Decorated Lipid Bilayer Systems for Interrogating the Spatial Requirements underlying B cell Immune-Synapse Formation
Bebhinn Treanor, Yuchen Hou, Chung -Yi Tseng, Raysa Orin, Emily Cohen and Leo Chou
