Cell Death: Part F, Volume 211 presents the latest advances and comprehensive reviews in the rapidly evolving field of cell death. This volume explores Methods to Assess BAX Conformation, Activation, Translocation, and Mitochondrial Outer Membrane Permeabilization, Proximity biotin labelling identification of Bcl-2 protein interactions in live cells, Bimolecular fluorescence complementation assays for investigating Bcl-2 family protein interactions, Assessing apoptotic sensitivity in primary mouse B cells by BH3 profiling, Liposome permeabilization assays to monitor pore formation by cell death proteins, Flow Cytometry Assessment of Mitochondrial Membrane Potential, Biomass, and ROS in OXPHOS-Dependent and Glycolytic Cancer Cells during Cell Death Induction, and much more.
Additional chapters cover Assessment of Viability Versus Cell Death: What is What?, Flow cytometry-based drug response profiling of primary B-precursor ALL cells in co-culture with bone marrow-derived stromal cells, An in vitro model of efferocytosis in macrophage foam cells to probe lipid-driven dysregulation of dead cell clearance, Measuring tumor cell killing using in vivo bioluminescence imaging, and more.
Additional chapters cover Assessment of Viability Versus Cell Death: What is What?, Flow cytometry-based drug response profiling of primary B-precursor ALL cells in co-culture with bone marrow-derived stromal cells, An in vitro model of efferocytosis in macrophage foam cells to probe lipid-driven dysregulation of dead cell clearance, Measuring tumor cell killing using in vivo bioluminescence imaging, and more.
Table of Contents
1. Methods to Assess BAX Conformation, Activation, Translocation, and Mitochondrial Outer Membrane PermeabilizationEvripidis Gavathiotis and Deniz Gulfem Ozturk
2. Proximity biotin labelling identification of Bcl-2 protein interactions in live cells.
Andrew Gilmore
3. Bimolecular fluorescence complementation assays for investigating Bcl-2 family protein interactions
Isabel Marzo, Andrea Bened�, Oscar Gonzalo, Laura Vela and Javier Naval
4. Assessing apoptotic sensitivity in primary mouse B cells by BH3 profiling
Joel Riley
5. Liposome permeabilisation assays to monitor pore formation by cell death proteins
Peter Czabotar, Michelle S. Miller, Katherine Davies and Ahmad Wardak
6. Flow Cytometry Assessment of Mitochondrial Membrane Potential, Biomass, and ROS in OXPHOS-Dependent and Glycolytic Cancer Cells during Cell Death Induction
Nikita Markov and Hans-Uwe Simon
7. Assessment of Viability Versus Cell Death: What is What?
Boris Zhivotovsky, Gelina S. Kopeina, Mikhail S. Chesnokov, Nikolay V. Pervushin and Maria A. Yapryntseva
8. Flow cytometry-based drug response profiling of primary B-precursor ALL cells in co-culture with bone marrow-derived stromal cells
Klaus Debatin, Julia Zinngrebe, Alexandra Niedermayer and L�der-Hinrich Meyer
9. An in vitro model of efferocytosis in macrophage foam cells to probe lipid-driven dysregulation of dead cell clearance
Kha Thanh Phan, Thu Uyen Tran, Mark Hulett and Tien Nguyen
10. Measuring tumor cell killing using in vivo bioluminescence imaging
Victor Peperzak
