Methods in Enzymology, Volume 599 is the second of two volumes focused on Fe-S cluster enzymes. Topics of interest in this new release include steps towards understanding mitochondrial Fe/S cluster biogenesis, iron sulfur clusters in zinc finger proteins, electrochemistry of Iron-sulfur enzymes, NRVS for Fe in biology and its experiment and basic interpretation,
methods for studying iron regulatory protein 1, an important protein in human iron metabolism, the characterization of glutaredoxin Fe-S cluster binding interactions using circular dichroism spectroscopy, fluorescent reporters to track Fe-S cluster assembly and transfer reactions, methods for studying the Fe-S cluster containing base excision repair glycosylase MUTYH, and more.
- Contain contributions from leading authorities on enzymology
- Informs and updates on all the latest developments in the field
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1. Steps Towards Understanding Mitochondrial Fe/S Cluster Biogenesis Dennis R. Winge 2. Iron Sulfur Clusters in Zinc Finger Proteins Sarah Michel 3. Electrochemistry of Iron-sulfur Enzymes Fraser Armstrong 4. NRVS for Fe in Biology: Experiment and Basic Interpretation Leland Bruce Gee 5. Methods for studying Iron Regulatory Protein 1: an important protein in human iron metabolism Tracey Rouault 6. Characterization of glutaredoxin Fe-S cluster binding interactions using circular dichroism spectroscopy Caryn E. Outten 7. Conformationally gated electron transfer in nitrogenase. Isolation, purification and characterization of nitrogenase from Gluconacetobacter diazotrophicus F. Akif Tezcan and Cedric P. Owens 8. Fluorescent reporters to track Fe-S cluster assembly and transfer reactions David Barondeau 9. Methods for studying the Fe-S cluster containing base excision repair glycosylase MUTYH Sheila David 10. Advanced X-ray Spectroscopic Methods for Studying Iron-Sulfur-Containing Proteins and Model Complexes Serena DeBeer 11. Chemical and biochemical reconstitution of iron-sulfur cluster assembly Roland Lill 12. Biochemical analyses of human iron-sulfur protein biogenesis and of related diseases Roland Lill 13. Nbp35-Cfd1 Iron sulfur cluster scaffold: a case study in employing fluorescent nucleotide probes to understand NTPase for metallocofactor biosynthesis Deborah Perlstein 14. Methods for efficient large-scale production, purification, and structure determinations of Recombinant Fe-S cluster proteins John Tainer