Mitosis and Meiosis Part B, Vol 145. Methods in Cell Biology

  • ID: 4455059
  • Book
  • 390 Pages
  • Elsevier Science and Technology
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Mitosis and Meiosis, Part B, Volume 145, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Mitotic live cell imaging at different time scales, the characterization of mitotic spindle by multi-mode correlative microscopy, STED microscopy of mitosis, Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture, quantification of three-dimensional spindle architecture, Imaging based assays for mitotic chromosome condensation and dynamics, and more.

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on both mitosis and meiosis
  • Includes relevant, analysis based topics

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Helder Maiato and Melina Schuh

1. Mitotic live-cell imaging at different timescales

Anna-Maria Olziersky, Chris A. Smith, Nigel Burroughs, Andrew D. McAinsh and Patrick Meraldi

2. Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture

Nicholas I. Clarke and Stephen J. Royle

3. Quantification of three-dimensional spindle architecture

Norbert Lindow, Stefanie Redemann, Florian Brünig, Gunar Fabig, Thomas Müller-Reichert and Steffen Prohaska

4. Quantitative live and super-resolution microscopy of mitotic chromosomes

Nike Walther and Jan Ellenberg

5. Live imaging of cell division in 3D stem-cell organoid cultures

Ana C.F. Bolhaqueiro, Richard H. van Jaarsveld, Bas Ponsioen, René M. Overmeer, Hugo J. Snippert and Geert J.P.L. Kops

6. Choosing the right microscope to image mitosis in zebrafish embryos: A practical guide

Iskra Yanakieva, Marija Matejcic and Caren Norden

7. Advanced microscopy methods for bioimaging of mitotic microtubules in plants

Petra Vyplelová, Miroslav Ovecka, George Komis and Jozef Samaj

8. Microsurgery and microinjection techniques in mitosis research

Charles A. Day, Jessica Hornick, Alyssa Langfald, Christopher Mader and Edward H. Hinchcliffe

9. Analyzing the micromechanics of the cell division apparatus

Yuta Shimamoto and Tarun M. Kapoor

10. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells

Ana Milas, Mihaela Jagric, Jelena Martincic and Iva M. Tolic

11. Live imaging of C. elegans oocytes and early embryos

Kimberley Laband, Benjamin Lacroix, Frances Edwards, Julie C. Canman and Julien Dumont

12. Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes

Pierre Romé and Hiroyuki Ohkura

13. Methods to study meiosis in insect spermatocytes

James R. LaFountain, Jr and Christopher S. Cohan

14. Live-cell microscopy of meiosis in spermatocytes

Hiroki Shibuya and Yoshinori Watanabe

15. Live imaging of cell division in preimplantation mouse embryos using inverted light-sheet microscopy

Judith Reichmann, Manuel Eguren, Yu Lin, Isabell Schneider and Jan Ellenberg

16. Correlated light and electron microscopy of cell division in large marine oocytes, eggs, and embryos

Mariia Burdyniuk, Natalia Wesolowska, Michal Fleszar, Matthia A. Karreman, Pedro Machado, Joana Borrego-Pinto, Bernhard Ruthensteiner, Yannick Schwab and Péter Lénárt

17. A microscopy-based approach for studying meiosis in live and fixed human oocytes

Agata P. Zielinska and Melina Schuh

18. Superresolution imaging of the synaptonemal complex

Katharina Schücker, Markus Sauer and Ricardo Benavente

19. Cryoelectron tomography of SYCP3 fibres under native conditions

Daniel Bollschweiler, Laura Radu and Luca Pellegrini
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