Mitosis and Meiosis Part A, Vol 144. Methods in Cell Biology

  • ID: 4465317
  • Book
  • 494 Pages
  • Elsevier Science and Technology
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Mitosis and Meiosis, Part A, Volume 144, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Analyzing the Spindle Assembly Checkpoint in human cell culture, an Analysis of CIN, a Functional analysis of the tubulin code in mitosis, Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis, Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cells, Small Molecule Tools in Mitosis Research, Optogenetic control of mitosis with photocaged chemical, and more.

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on both mitosis and meiosis
  • Includes relevant, analysis based topics

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Preface

Helder Maiato and Melina Schuh

- Assays for the spindle assembly checkpoint in cell culture

Chiara Marcozzi and Jonathon Pines

- Quantitative methods to measure aneuploidy and chromosomal instability

Kristina M. Godek and Duane A. Compton

- Dissecting the role of the tubulin code in mitosis

Luísa T. Ferreira, Ana C. Figueiredo, Bernardo Orr, Danilo Lopes and Helder Maiato

- Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis

Kara L. McKinley

- Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells

Bramwell G. Lambrus, Tyler C. Moyer and Andrew J. Holland

- Small molecule tools in mitosis research

Franziska Teusel, Lars Henschke and Thomas U. Mayer

- Optogenetic control of mitosis with photocaged chemical dimerizers

Huaiying Zhang, David M. Chenoweth and Michael A. Lampson

- Measuring mitotic forces

Anna A. Ye and Thomas J. Maresca

- Employing the one-cell C. elegans embryo to study cell division processes

Neil Hattersley, Pablo Lara-Gonzalez, Dhanya Cheerambathur, Sebastian Gomez, Taekyung Kim, Bram Prevo, Renat Khaliullin, Kian-Yong Lee, Midori Ota, Rebecca Green, Karen Oegema and Arshad Desai

- A cell-free system of Drosophila egg explants supporting native mitotic cycles

Jorge de-Carvalho, Ojas Deshpande, Catarina Nabais and Ivo A. Telley

- Living Xenopus oocytes, eggs, and embryos as models for cell division

Ani Varjabedian, Angela Kita and William Bement

- Spreading of chromosomes from mouse oocytes and mammalian cultured cells for light microscopic analysis

Mariana C.C. Silva, Gordana Wutz, Kikuë Tachibana and Jan-Michael Peters

- In vitro reconstitution of lateral to end-on conversion of kinetochore-microtubule attachments

Manas Chakraborty, Ekaterina Tarasovetc and Ekaterina L. Grishchuk

- Isolation of mitotic chromosomes from vertebrate cells and characterization of their proteome by mass spectrometry

Itaru Samejima and William C. Earnshaw

- Purification of kinetochores from the budding yeast Saccharomyces cerevisiae

Amitabha Gupta, Rena K. Evans, Lori B. Koch, Aimee J. Littleton and Sue Biggins

- Characterization of DNA helicases and nucleases from meiotic extracts of S. cerevisiae

Rokas Grigaitis, Aitor Susperregui, Philipp Wild and Joao Matos

- Single-nucleus Hi-C of mammalian oocytes and zygotes

Johanna Gassler, Ilya M. Flyamer and Kikuë Tachibana

- Induction of fetal primary oocytes and the meiotic prophase from mouse pluripotent stem cells

Hidetaka Miyauchi, Hiroshi Ohta and Mitinori Saitou

- Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos

Greg FitzHarris, John Carroll and Karl Swann

- Single cell genomics to study DNA and chromosome changes in human gametes and embryos

Robert C. Blanshard, Chongyi Chen, X. Sunney Xie and Eva R. Hoffmann

- Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes

Hirohisa Kyogoku, Shuhei Yoshida and Tomoya S. Kitajima
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