Echinoderms, Volume 150 in the Methods in Cell Biology series, highlights new advances in the field, with this update presenting interesting chapters on Procuring animals and culturing of eggs and embryos, Cryopreservation of sea urchin gametes, Culturing of Amphiura filiformis as a model for gene expression studies, Temnopleurus as an emerging echinoderm model, Culturing of sand dollars, cidaroids and heart urchins, Culturing and genomics of Lytechinus pictus, Culturing echinoderm larvae through metamorphosis, Microinjection methods, Injection of exogenous messages and protein overexpression, Blastomere transplantation, Cell isolation, Visualizing embryonic polarity, and much more.
- Clear, concise protocols provided by experts who have established the echinoderms as a model systems
- Highlights new advances in the field, with this update presenting interesting chapters on echinoderms
Procurement and Culturing of Established and Emerging Echinoderm Models 1. Procuring animals and culturing of eggs and embryos Nikki L. Adams, Kathy R. Foltz, Andreas Heyland and Linda Rice 2. Cryopreservation of sea urchin gametes Serean Adams and Estefania Paredes 3. Culturing of Amphiura filiformis as a model for gene expression studies Paola Oliveri 4. Temnopleurus as an emerging echinoderm model Shunsuke Yaguchi 5. Culturing of sand dollars, cidaroids and heart urchins Takuya Minokawa, Taku Hibino and Atsuko Yamazaki 6. Culturing and genomics of Lytechinus pictus Deirdre Lyons 7. Culturing echinoderm larvae through metamorphosis Jason Hodin
Experimental embryology approaches 8. Microinjection methods Junko Yaguchi 9. Injection of exogenous messages and protein overexpression George von Dassow 10. Blastomere transplantation D R. McClay 11. Cell isolation Gary M. Wessel 12. Visualizing embryonic polarity Athula Wikramanayake 13. Methods for the isolation, labeling and live-imaging and analysis of echinoderm primordial germ cells Joseph Paul Campanale and Amro Hamdoun 14. Methods for studying skeletogenesis Brian Livingston
Approaches for assessing environmental influences on adults and embryos 15. Larval immune cell approaches Jon Rast 16. Methods for collection, handling, and analysis of sea urchin coelomocytes L. Courtney Smith 17. Methods and best practices for assessing the effects of pH on fertilization and development of sea urchins Sam DuPont and Meike Stump 18. Methods and best practices for toxicology studies in echinoderm embryos and larvae Gary N. Cherr
Sea urchins in the classroom 19. Sea urchins in the classroom David Epel and Victor Vacquier 20. A laboratory on xenobiotic transporter activity Lauren E. Shipp 21. Sea urchin labs. Laura Romano, Robert Morris and Christine Byrum 22. Analyzing gene expression in sea star embryos David Carroll