Methods in Kidney Cell Biology, Volume 153, represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, and clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics - a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.
- Written by experts in their field who have perfected the methods they write about
- Covers a wide range of topics, from state-of-the-art techniques that may require specialized equipment, to tried-and-true classic methods in their most refined form
- Includes cutting-edge, recently developed methods
1. Single glomerular proteomics
a novel method in translational glomerular cell biology Markus Rinschen 2. Measurement of cytosolic and intraciliary calcium in live cells Surya Nauli 3. Differentiation of human kidney organoids from pluripotent stem cells Benjamin S. Freedman 4. Quantifying autophagic flux in kidney tissue using structured illumination microscopy Craig Robert Brooks 5. Generation of primary cells from ADPKD and normal human kidneys Darren Wallace 6. ADPKD cell proliferation and Cl-dependent fluid secretion Darren Wallace 7. In vitro cyst formation of ADPKD cells Darren Wallace 8. Analysis of primary cilia in renal tissue and cells Pamela Tran 9. Intravital visualization of cilia in the kidney Bradley K. Yoder 10. Nephrocytes as a model to study podocyte injury Paul Brinkkötter 11. Studying Na and K channels in aldosterone-sensitive distal nephrons Wenhui Wang 12. Novel fluorescence techniques to quantitate renal cell biology János Peti-Peterdi 13. Application of physiological shear stress to renal tubular epithelial cells Nick Ferrell 14. Metanephric organ culture Kim Calvet 15. Investigation of epigenetics in kidney cell biology Xiaogang Li 16. In vivo analysis of renal epithelial cells in zebrafish Zhaoxia Sun 17. Kidney harvesting and metabolite extraction for metabolomics studies in rodents Maria V. Irazabal 18. Measuring calcium and cAMP in renal epithelial cells under static and fluid shear stress using genetically encoded biosensors Fouad Chebib 19. Manipulating renal gene expression in vivo using oligonucleotides Vishal D. Patel 20. Structural and Functional Characterization of the Polycystic Kidney by Magnetic Resonance Imaging Tim Kline