Methods in Kidney Cell Biology, Volume 153, represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, and clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics - a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.
- Written by experts in their field who have perfected the methods they write about
- Covers a wide range of topics, from state-of-the-art techniques that may require specialized equipment, to tried-and-true classic methods in their most refined form
- Includes cutting-edge, recently developed methods
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1. Generation of primary cells from ADPKD and normal human kidneys Darren P. Wallace and Gail A. Reif 2. Measurement of cytoplasmic and cilioplasmic calcium in a single living cell Rinzhin T. Sherpa, Rajasekharreddy Pala, Ashraf M. Mohieldin and Surya M. Nauli 3. Application of physiological shear stress to renal tubular epithelial cells Nicholas Ferrell, Ruben M. Sandoval, Bruce A. Molitoris, Paul Brakeman, Shuvo Roy and William H. Fissell 4. ADPKD cell proliferation and Cl--dependent fluid secretion Gail A. Reif and Darren P. Wallace 5. In vitro cyst formation of ADPKD cells Madhulika Sharma, Gail A. Reif and Darren P. Wallace 6. Engineering kidney tissues for polycystic kidney disease modeling and drug discovery Valerio Brizi, Valentina Benedetti, Angelo Michele Lavecchia and Christodoulos Xinaris 7. Differentiation of human kidney organoids from pluripotent stem cells Nelly M. Cruz and Benjamin S. Freedman 8. Studying Na+ and K+ channels in aldosterone-sensitive distal nephrons Jacques Teulon and Wen-Hui Wang 9. Metanephric organ culture Robin L. Maser, Brenda S. Magenheimer and James P. Calvet 10. Ex vivo kidney slice preparations as a model system to study signaling cascades in kidney epithelial cells Biagio Saitta, Michael F. Jalili, Hamidreza Zohoorkari, Renee Rao, Kenneth R. Hallows, Catherine J. Baty and Nuria M. Pastor-Soler 11. Analysis of primary cilia in renal tissue and cells Luciane M. Silva, Wei Wang, Bailey A. Allard, Tana S. Pottorf, Damon T. Jacobs and Pamela V. Tran 12. Quantifying autophagic flux in kidney tissue using structured illumination microscopy Kensei Taguchi, Bertha C. Elias, Subo Qian and Craig R. Brooks 13. Investigation of epigenetics in kidney cell biology Linda Xiaoyan Li, Ewud Agborbesong, Lu Zhang and Xiaogang Li