In recent years, the field of epigenetics has grown significantly, driving new understanding of human developmental processes and disease expression, as well as advances in diagnostics and therapeutics. As the field of epigenetics continues to grow, methods and technologies have multiplied, resulting in a wide range of approaches and tools researchers might employ.
Epigenetics Methods offers comprehensive instruction in methods, protocols, and experimental approaches applied in field of epigenetics. Here, across thirty-five chapters, specialists offer step-by-step overviews of methods used to study various epigenetic mechanisms, as employed in basic and translational research. Leading the reader from fundamental to more advanced methods, the book begins with thorough instruction in DNA methylation techniques and gene or locus-specific methylation analyses, followed by histone modification methods, chromatin evaluation, enzyme analyses of histone methylation, and studies of non-coding RNAs as epigenetic modulators. Recently developed techniques and technologies discussed include single-cell epigenomics, epigenetic editing, computational epigenetics, systems biology epigenetic methods, and forensic epigenetic approaches. Epigenetics methods currently in-development, and their implication for future research, are also considered in-depth.
In addition, as with the wider life sciences, reproducibility across experiments, labs, and subdisciplines is a growing issue for epigenetics researchers. This volume provides consensus-driven methods instruction and overviews. Tollefsbol and contributing authors survey the range of existing methods; identify best practices, common themes, and challenges; and bring unity of approach to a diverse and ever-evolving field.
- Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application
- Integrates technology and translation with fundamental chapters on epigenetics methods, as well as chapters on more novel and advanced epigenetics methods
- Written at verbal and technical levels that can be understood by scientists and students alike
- Includes chapters on state-of-the-art techniques such as single-cell epigenomics, use of CRISPR/Cas9 for epigenetic editing, and epigenetics methods applied to forensics
2. Disease in a dish: Cellular models to understand human conditions
3. The Yeast, a versatile model system to study human disease
4. The worm, how can it help?
5. Understanding human genetic disease with the fly
6. The fish as a model for human diseases
7. The mouse model
8. Large animal models of rare genetic disorders
9. Crisper/Cas technology
10. Integrative modeling and novel technologies in human genomics
Professor of Biology, University of Alabama at Birmingham, Birmingham, AL. Dr. Tollefsbol is a Professor of Biology and a Senior Scientist in the Center for Aging, Comprehensive Cancer Center, Nutrition Obesity Research Center, and the Comprehensive Diabetes Center at the University of Alabama at Birmingham (UAB). He is Director of the UAB Cell Senescence Culture Facility which he established in 1999 and a Steering Committee Member of the UAB Center for Aging. Dr. Tollefsbol trained as a Postdoctoral Fellow and Assistant Research Professor with members of the National Academy of Science at Duke University and the University of North Carolina. He earned doctorates in molecular biology and osteopathic medicine from the University of North Texas Health Sciences Center and his bachelor's degree in Biology from the University of Houston. He has received prior funding from the NIA, NCI, NHLBI, NIMH and other federal institutes as well as the Glenn Foundation for Medical Research, Susan G. Komen for the Cure, the American Federation for Aging Research (AFAR), and the American Institute for Cancer Research (AICR) among many other sources. The Glenn Foundation for Medical Research funding was unsolicited and was awarded to Dr. Tollefsbol for lifetime contributions to the field of aging. In 2006 Dr. Tollefsbol was selected and highlighted as part of the 25th anniversary of the AFAR for significant contributions to aging research. Dr. Tollefsbol's research interests have covered a wide range of topics such as aging, epigenetics, nutrition, cancer, telomerase, and caloric restriction. Work from his laboratory has been featured in Women's Health magazine, Shape magazine, and the AICR Newsletter and Dr. Tollefsbol has been a Scientist in the Spotlight in ScienceNow. Currently he serves as an Associate Editor for Frontiers in Epigenomics and is on the Editorial Boards of the international journals Open Longevity Science, Epigenetics of Diabetes and Obesity, Molecular Biotechnology and Clinical Epigenetics. He is also a contributing Editor of Lewin's GENES X classic textbook. Over 25 of the publications from Dr. Tollefsbol's laboratory have received national or international accolades such as best paper award, selection for press release by the journal editors and featured on the journal homepage. Dr. Tollefsbol has been invited to give presentations on his research in many countries including Germany, China, Italy, Switzerland, France and The Netherlands as well as at various leading institutions in the US such as Harvard Medical School, Tufts University and the University of California at San Francisco. His research has received considerable media attention both nationally and internationally through television, newspaper and radio venues and has been highlighted in eScience News and ScienceDaily. He has ten books which have been published or are in progress and a recent book on "Epigenetics of Aging that Dr. Tollefsbol co-authored and edited was highlighted in Nature.