Proteolytic Enzymes and their Inhibitors in Infectious Pathogens. Foundations and Frontiers in Enzymology

Table of Contents

Part 1: Introduction
1. Overview of proteases of pathogenic organisms
2. Introducing endogenous macromolecular protease inhibitors of pathogenic organisms

Part 2: New methods to identify protease substrates and inhibitors
3. DNA-linked inhibitor antibody assay (DIANA) to capture proteases in complex mixtures and identify novel small-molecule inhibitors
4. New advances in positional proteomics strategies to study protein processing
5. Bioinformatics approaches for accurate prediction of protease-specific substrates and their cleavage sites
6. Delineating peptidase specificities at the host-parasite interface using Multiplex Substrate Profiling by Mass Spectrometry (MSP-MS)
7. Recent Advances in Applications of Proteomic Approaches and Chemical Tools for Pathogen Profiling
8. Methods for mapping the mutational landscape of virus protease inhibitor binding sites to predict drug resistance

Part 3: Aspartic proteases and their inhibitors
9. Bacterial signal peptidases
10. Plasmepsins of the malaria parasite Plasmodium
11. Aspartic proteases of kinetoplasts
12. HIV1 subtype C protease hinge region dynamics and flap flexibility in drug resistance
13. Aspartic protease inhibitors of nematodes

Part 4: Cysteine proteases and their inhibitors
14. Polyprotein processing in SARS Cov-2
15. Gingipains of Porphyromonas gingivalis deactivators of innate immune and inflammatory defense mechanisms
16. Sortases in pilus biogenesis of Gram-positive bacteria
17. Cysteine proteases of protozoa
18. Multifunctional Nidovirus papain-like proteases
19. Chagasins: Inhibitors of Cysteine Proteases in Protozoa
20. Targeting the 3C-like proteases of Picornaviruses, Noroviruses, and Coronaviruses

Part 5: Metalloproteases and their inhibitors
21. The FusC about proteases involved in microbial iron piracy
22. Metallo-aminopeptidases in parasites and bacteria

Part 6: Serine proteases and their inhibitors
23. Oligopeptidase B popping up in many pathogens as a virulence factor
24. Ecotins of bacteria and protozoa
25. Serpins of trematodes and nematodes
26. Flaviviral serine NS2B-NS3 endoproteases

Part 7: Threonine proteases
27. Proteasome in Plasmodium and kinetoplast parasites
28. Proteasome in infectious organisms

Authors

Theresa Coetzer Professor, (SARChI) Chair, Biochemistry Department, University of KwaZulu-Natal, Pietermaritzburg, South Africa. Professor Theresa Coetzer's research focusses on proteolytic enzymes as diagnostic and drug targets in African parasitic diseases: mainly animal and human trypanosomiasis (nagana and sleeping sickness) and also trichinellosis and theileriosis. She also has expertise in producing antibodies in chickens against the whole proteases and against peptides corresponding to epitopes identified in silico. Dr. Coetzer holds a DST-NRF South African Research Chair Initiative (SARChI), as Chair in Proteolysis in Haemostasis, Health and Disease.