Automated Next-Generation Sequencing (NGS) Library Preparation Market Report 2025

The automated next-generation sequencing (NGS) library preparation market size has grown rapidly in recent years. It will grow from $0.63 billion in 2024 to $0.72 billion in 2025 at a compound annual growth rate (CAGR) of 13.8%. The growth in the historic period can be attributed to increasing adoption for infectious disease surveillance, rising funding for genomic research, expanded implementation during pandemics, growing accessibility of NGS platforms in research laboratories, and rising applications in precision medicine.

The automated next-generation sequencing (NGS) library preparation market size is expected to see rapid growth in the next few years. It will grow to $1.2 billion in 2029 at a compound annual growth rate (CAGR) of 13.4%. The growth in the forecast period can be attributed to increasing adoption in clinical diagnostics, rising demand for personalized medicine, expanding applications in oncology research, growing need for high-throughput sequencing, and heightened focus on reducing turnaround times. Major trends in the forecast period include improvements in workflow efficiency, integration of artificial intelligence for data analysis, technology-driven protocol standardization, development of user-friendly sequencing systems, and advancements in cloud-based data management solutions.

The rise in gene therapy is expected to propel the growth of the automated next-generation sequencing (NGS) library preparation market going forward. Gene therapy refers to treatments that modify a person’s genes to cure or prevent diseases. Its adoption is increasing due to the rising prevalence of genetic disorders requiring targeted and long-lasting interventions. Automated NGS library preparation supports gene therapy by preparing high-quality DNA or RNA samples for sequencing, enabling accurate analysis and validation of genetic modifications. For instance, in April 2024, according to the American Society of Gene and Cell Therapy, a US-based non-profit medical organization, the number of phase III gene therapy clinical trials rose by 10% in the fourth quarter, marking the first quarterly increase since Q3 2022. Therefore, the rise in gene therapy is driving the growth of the automated NGS library preparation market.

Key companies in the automated NGS library preparation market are focusing on advanced technologies, such as Xpandomer technology, to improve sequencing speed, data accuracy, and workflow scalability, thereby enabling more efficient genomic analysis. Xpandomer technology converts target DNA or RNA molecules into synthetic expanded polymers approximately 50 times longer than the original strand, enhancing sequencing resolution. For example, in February 2025, Roche AG, a Switzerland-based biotechnology company, launched Sequencing by Expansion, an automated NGS platform designed to support advanced diagnostics, disease research, and precision medicine. The platform enables precise analysis of genomic alterations across cancers and rare genetic disorders, improving data quality and enabling deeper biological insights.

In January 2023, Agilent Technologies Inc., a US-based biotechnology company, acquired Avida Biomed to enhance its precision oncology capabilities. The acquisition integrates Avida Biomed’s high-performance NGS target enrichment workflows with Agilent’s platforms, strengthening genomic analysis for applications such as biomarker discovery and therapy selection. Avida Biomed is a US-based biotechnology company specializing in NGS tools for cancer research.

Major players in the automated next-generation sequencing (NGS) library preparation market are F. Hoffmann-La Roche Ltd., Thermo Fisher Scientific Inc., Agilent Technologies India Private Limited, Illumina Inc., Beckman Coulter Inc., Bio-Rad Laboratories Inc., IDT Corporation, Tecan Group AG, Hamilton Company, Takara Bio Inc., 10x Genomics Inc., Oxford Nanopore Technologies plc, Twist Bioscience Corporation, New England Biolabs Inc., SPT Labtech Limited, Watchmaker Genomics Inc., Pillar Biosciences Inc., Aurora Biomed Inc., BGI Group, SEQWELL INC., and Hudson Robotics Inc.

North America was the largest region in the automated next-generation sequencing (NGS) library preparation market in 2024. Asia-Pacific is expected to be the fastest-growing region in the forecast period. The regions covered in automated next-generation sequencing (NGS) library preparation market report are Asia-Pacific, Western Europe, Eastern Europe, North America, South America, Middle East and Africa. The countries covered in the automated next-generation sequencing (NGS) library preparation market report are Australia, Brazil, China, France, Germany, India, Indonesia, Japan, Russia, South Korea, UK, USA, Canada, Italy, Spain.

Note that the outlook for this market is being affected by rapid changes in trade relations and tariffs globally. The report will be updated prior to delivery to reflect the latest status, including revised forecasts and quantified impact analysis. The report’s Recommendations and Conclusions sections will be updated to give strategies for entities dealing with the fast-moving international environment.

The sudden escalation of U.S. tariffs and the consequent trade frictions in spring 2025 are severely impacting the healthcare sector, particularly in the supply of critical medical devices, diagnostic equipment, and pharmaceuticals. Hospitals and healthcare providers are facing higher costs for imported surgical instruments, imaging equipment, and consumables such as syringes and catheters, many of which have limited domestic alternatives. These increased costs are straining healthcare budgets, leading some providers to delay equipment upgrades or pass on expenses to patients. Additionally, tariffs on raw materials and components are disrupting the production of essential drugs and devices, causing supply chain bottlenecks. In response, the industry is diversifying sourcing strategies, boosting local manufacturing where possible, and advocating for tariff exemptions on life-saving medical products.

Automated next-generation sequencing (NGS) library preparation refers to the use of robotic systems and software to perform the complex and repetitive steps involved in preparing DNA or RNA samples for sequencing. This process includes fragmentation, end-repair, adapter ligation, and amplification, ensuring consistency, scalability, and reduced human error in high-throughput sequencing workflows.

The primary types of automated next-generation sequencing (NGS) library preparation are targeted genome sequencing, whole genome sequencing, whole exome sequencing, and others. Targeted genome sequencing focuses on analyzing specific genomic regions of interest, offering high-depth coverage and cost-effective mutation detection for both diagnostic and research applications. The workflow involves steps such as nucleic acid extraction, fragmentation, adapter ligation, purification, and quantification. These processes are widely applied in drug and biomarker discovery, disease diagnostics, and others by several end users, including hospitals and clinics, academic and research institutions, pharmaceutical and biotechnology companies, and others.

The automated next-generation sequencing (NGS) library preparation market research report is one of a series of new reports that provides automated next-generation sequencing (NGS) library preparation market statistics, including automated next-generation sequencing (NGS) library preparation industry global market size, regional shares, competitors with a automated next-generation sequencing (NGS) library preparation market share, detailed automated next-generation sequencing (NGS) library preparation market segments, market trends and opportunities, and any further data you may need to thrive in the automated next-generation sequencing (NGS) library preparation industry. This automated next-generation sequencing (NGS) library preparation market research report delivers a complete perspective of everything you need, with an in-depth analysis of the current and future scenario of the industry.

The automated next-generation sequencing (NGS) library preparation market consists of revenues earned by entities by providing services such as sample processing automation, quality control, and workflow optimization solutions. The market value includes the value of related goods sold by the service provider or included within the service offering. The automated next-generation sequencing (NGS) library preparation market also includes sales of workstations, robotic liquid handlers, and others. Values in this market are ‘factory gate’ values, that is the value of goods sold by the manufacturers or creators of the goods, whether to other entities (including downstream manufacturers, wholesalers, distributors and retailers) or directly to end customers. The value of goods in this market includes related services sold by the creators of the goods.

The market value is defined as the revenues that enterprises gain from the sale of goods and/or services within the specified market and geography through sales, grants, or donations in terms of the currency (in USD unless otherwise specified).

The revenues for a specified geography are consumption values that are revenues generated by organizations in the specified geography within the market, irrespective of where they are produced. It does not include revenues from resales along the supply chain, either further along the supply chain or as part of other products.

This product will be delivered within 3-5 business days.