Next-Generation Sequencing Library Preparation Market Report 2025

The next-generation sequencing library preparation market size has grown rapidly in recent years. It will grow from $1.42 billion in 2024 to $1.63 billion in 2025 at a compound annual growth rate (CAGR) of 14.7%. The growth during the historic period can be credited to the expanding use of NGS library preparation in infectious disease surveillance, increasing financial support for genomic research, widespread adoption during the pandemic, improved accessibility for research laboratories, and the rising application of precision medicine.

The next-generation sequencing library preparation market size is expected to see rapid growth in the next few years. It will grow to $2.78 billion in 2029 at a compound annual growth rate (CAGR) of 14.3%. The growth in the forecast period can be attributed to the rising adoption of NGS library preparation in clinical diagnostics, increasing demand for personalized medicine, expanding applications in oncology research, growing need for high-throughput sequencing, and heightened focus on minimizing turnaround times. Key trends expected during this period include improvements in workflow efficiency, integration of artificial intelligence for advanced data analysis, technology-driven standardization of protocols, development of more user-friendly systems, and advancements in cloud-based data management solutions.

The growing adoption of gene therapy is expected to drive the next-generation sequencing (NGS) library preparation market in the coming years. Gene therapy, which modifies a person’s genes to prevent or treat diseases, is gaining momentum due to the rising prevalence of genetic disorders that require targeted, long-lasting interventions. NGS library preparation plays a crucial role in gene therapy by enabling accurate analysis and validation of genetic modifications through the preparation of high-quality DNA or RNA samples for sequencing. For example, according to the American Society of Gene and Cell Therapy, a US-based non-profit medical and scientific organization, the number of phase III gene therapy clinical trials increased by 10% in the fourth quarter of 2024, marking the first quarterly rise since the third quarter of 2022. Consequently, the growth of gene therapy is fueling the NGS library preparation market.

Companies in the NGS library preparation market are focusing on advanced technologies such as Xpandomer technology to enhance sequencing speed, improve data accuracy, and increase scalability, streamlining workflows for more efficient and reliable genomic analysis. Xpandomer technology converts a target DNA or RNA molecule into a synthetic expanded polymer called an Xpandomer, which is 50 times longer than the original strand. For instance, in February 2025, Roche AG, a Switzerland-based biotechnology company, launched Sequencing by Expansion, a next-generation sequencing platform designed to improve genomic resolution for advanced diagnostics, disease research, and precision medicine. This automated NGS library preparation system enhances data quality, accelerates genomic analysis, and allows precise detection of genomic alterations across a wide range of diseases, including cancer and rare genetic disorders.

In January 2023, Agilent Technologies Inc., a US-based biotechnology company, acquired Avida Biomed for an undisclosed amount. This acquisition strengthens Agilent’s capabilities in cancer research and diagnostics by integrating advanced NGS tools with its existing platforms, enhancing precision medicine applications such as biomarker discovery and therapy selection. Avida Biomed is a US-based biotechnology firm specializing in high-performance NGS target enrichment workflows for cancer research.

Major players in the next-generation sequencing library preparation market are F. Hoffmann-La Roche Ltd., Thermo Fisher Scientific Inc., Agilent Technologies India Private Limited, Illumina Inc., Beckman Coulter Inc., Bio-Rad Laboratories Inc., IDT Corporation, Tecan Group AG, Hamilton Company, Takara Bio Inc., 10x Genomics Inc., Oxford Nanopore Technologies Plc, Twist Bioscience Corporation, New England Biolabs Inc., SPT Labtech Limited, Watchmaker Genomics Inc., Pillar Biosciences Inc., Aurora Biomed Inc., BGI Group, and SEQWELL INC.

North America was the largest region in the next-generation sequencing library preparation market in 2024. Asia-Pacific is expected to be the fastest-growing region in the forecast period. The regions covered in next-generation sequencing library preparation market report are Asia-Pacific, Western Europe, Eastern Europe, North America, South America, Middle East and Africa. The countries covered in the next-generation sequencing library preparation market report are Australia, Brazil, China, France, Germany, India, Indonesia, Japan, Russia, South Korea, UK, USA, Canada, Italy, Spain.

Note that the outlook for this market is being affected by rapid changes in trade relations and tariffs globally. The report will be updated prior to delivery to reflect the latest status, including revised forecasts and quantified impact analysis. The report’s Recommendations and Conclusions sections will be updated to give strategies for entities dealing with the fast-moving international environment.

The fast surge in U.S. tariffs and the trade tensions that followed in spring 2025 are heavily affecting the medical equipment sector, particularly for imported imaging machine components, surgical-grade stainless steel, and plastic disposables. Hospitals and clinics resist price hikes, pressuring manufacturers’ margins. Regulatory hurdles compound the problem, as tariff-related supplier changes often require re-certification of devices, delaying time-to-market. Companies are mitigating risks by dual-sourcing critical parts, expanding domestic production of commoditized items, and accelerating R&D in cost-efficient materials.

Next-generation sequencing (NGS) library preparation refers to the process of preparing DNA or RNA samples for sequencing. This involves amplifying the genetic material and adding specific tags so that sequencing machines can accurately read and interpret the data. Proper library preparation is essential for ensuring high-quality and reliable results, enabling detailed studies of genes, diseases, and other biological processes.

The priamry types of NGS library preparation include targeted genome sequencing, whole genome sequencing, whole exome sequencing, and others. Targeted genome sequencing is especially valued for its high-depth coverage and cost-efficient detection of mutations in specific genomic regions, making it suitable for both research and diagnostic applications. Key products include reagents and consumables, DNA library preparation kits, RNA library preparation kits, and other related materials. The technologies supporting these preparations include ion semiconductor sequencing, sequencing by synthesis (SBS), nanopore sequencing, single-molecule real-time (SMRT) sequencing, and others. NGS library preparation finds applications in drug and biomarker discovery, disease diagnostics, cancer diagnostics, reproductive health diagnostics, infectious disease diagnostics, and more. End users include hospitals and clinics, academic and research institutions, pharmaceutical and biotechnology companies, among others.

The next-generation sequencing library preparation market research report is one of a series of new reports that provides next-generation sequencing library preparation market statistics, including the next-generation sequencing library preparation industry global market size, regional shares, competitors with the next-generation sequencing library preparation market share, detailed next-generation sequencing library preparation market segments, market trends, and opportunities, and any further data you may need to thrive in the next-generation sequencing library preparation industry. This next-generation sequencing library preparation market research report delivers a complete perspective of everything you need, with an in-depth analysis of the current and future scenarios of the industry.

The next-generation sequencing library preparation market consists of revenues earned by entities by providing services such as sample preparation and extraction, quantification and quality assessment, amplification and enrichment, quality control and validation, and barcode tagging. The market value includes the value of related goods sold by the service provider or included within the service offering. The next-generation sequencing (NGS) library preparation market also includes sales of automated liquid handling systems, thermal cyclers, fragmentation systems, and barcoding and sample tracking systems. Values in this market are ‘factory gate’ values, that is, the value of goods sold by the manufacturers or creators of the goods, whether to other entities (including downstream manufacturers, wholesalers, distributors, and retailers) or directly to end customers. The value of goods in this market includes related services sold by their creators.

The market value is defined as the revenues that enterprises gain from the sale of goods and/or services within the specified market and geography through sales, grants, or donations in terms of the currency (in USD unless otherwise specified).

The revenues for a specified geography are consumption values that are revenues generated by organizations in the specified geography within the market, irrespective of where they are produced. It does not include revenues from resales along the supply chain, either further along the supply chain or as part of other products.

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