The next-generation sequencing library preparation market size has grown rapidly in recent years. It will grow from $1.63 billion in 2025 to $1.86 billion in 2026 at a compound annual growth rate (CAGR) of 14.5%. The growth in the historic period can be attributed to limited sequencing throughput, high cost of library preparation kits, reliance on manual preparation workflows, growing demand for genomic research, increasing adoption of sequencing in clinical diagnostics.
The next-generation sequencing library preparation market size is expected to see rapid growth in the next few years. It will grow to $3.17 billion in 2030 at a compound annual growth rate (CAGR) of 14.2%. The growth in the forecast period can be attributed to development of automated library preparation platforms, growth of whole genome and exome sequencing, rising investment in personalized medicine, expansion of academic and research genomics projects, integration of ngs data with ai and cloud analytics. Major trends in the forecast period include rising demand for high-throughput sequencing technologies, expansion of ngs library preparation kits and reagents, increased focus on personalized medicine and biomarker discovery, growth in infectious disease and cancer diagnostics, adoption of automation in library preparation workflows.
The increasing adoption of gene therapy is expected to drive the growth of the next-generation sequencing (NGS) library preparation market in the coming years. Gene therapy involves modifying a person’s genes to treat or prevent diseases and is expanding due to the rising prevalence of genetic disorders that require targeted and durable treatment solutions. NGS library preparation supports gene therapy by enabling accurate analysis and validation of genetic modifications through the preparation of high-quality DNA or RNA samples for sequencing. For example, in March 2024, IQVIA, a U.S.-based healthcare intelligence and life sciences company, reported that in 2023, global spending on cell and gene therapies reached $5.9 billion, a 38% increase from 2022, even though it accounted for only 0.4% of the $1.6 trillion total global medicines expenditure. Consequently, the rise of gene therapy is fueling the growth of the NGS library preparation market.
Companies in the NGS library preparation market are focusing on advanced technologies such as Xpandomer technology to enhance sequencing speed, improve data accuracy, and increase scalability, streamlining workflows for more efficient and reliable genomic analysis. Xpandomer technology converts a target DNA or RNA molecule into a synthetic expanded polymer called an Xpandomer, which is 50 times longer than the original strand. For instance, in February 2025, Roche AG, a Switzerland-based biotechnology company, launched Sequencing by Expansion, a next-generation sequencing platform designed to improve genomic resolution for advanced diagnostics, disease research, and precision medicine. This automated NGS library preparation system enhances data quality, accelerates genomic analysis, and allows precise detection of genomic alterations across a wide range of diseases, including cancer and rare genetic disorders.
In January 2023, Agilent Technologies Inc., a US-based biotechnology company, acquired Avida Biomed for an undisclosed amount. This acquisition strengthens Agilent’s capabilities in cancer research and diagnostics by integrating advanced NGS tools with its existing platforms, enhancing precision medicine applications such as biomarker discovery and therapy selection. Avida Biomed is a US-based biotechnology firm specializing in high-performance NGS target enrichment workflows for cancer research.
Major companies operating in the next-generation sequencing library preparation market are F. Hoffmann-La Roche Ltd., Thermo Fisher Scientific Inc., Agilent Technologies India Private Limited, Illumina Inc., Beckman Coulter Inc., Bio-Rad Laboratories Inc., IDT Corporation, Tecan Group AG, Hamilton Company, Takara Bio Inc., 10x Genomics Inc., Oxford Nanopore Technologies Plc, Twist Bioscience Corporation, New England Biolabs Inc., SPT Labtech Limited, Watchmaker Genomics Inc., Pillar Biosciences Inc., Aurora Biomed Inc., BGI Group, SEQWELL INC.
North America was the largest region in the next-generation sequencing library preparation market in 2025. Asia-Pacific is expected to be the fastest-growing region in the forecast period. The regions covered in the next-generation sequencing library preparation market report are Asia-Pacific, South East Asia, Western Europe, Eastern Europe, North America, South America, Middle East, Africa. The countries covered in the next-generation sequencing library preparation market report are Australia, Brazil, China, France, Germany, India, Indonesia, Japan, Taiwan, Russia, South Korea, UK, USA, Canada, Italy, Spain.
Tariffs are impacting the next-generation sequencing library preparation market by increasing the cost of imported reagents, consumables, and sequencing kits. Segments such as DNA and RNA library preparation kits are particularly affected, with Asia-Pacific regions including China and India facing high import duties. This has led to higher production costs and potential delays in supply. On the positive side, tariffs are encouraging local manufacturing, development of alternative reagents, and investment in domestic supply chains, which may strengthen regional capabilities over time.
The next-generation sequencing library preparation market research report is one of a series of new reports that provides next-generation sequencing library preparation market statistics, including next-generation sequencing library preparation industry global market size, regional shares, competitors with a next-generation sequencing library preparation market share, detailed next-generation sequencing library preparation market segments, market trends and opportunities, and any further data you may need to thrive in the next-generation sequencing library preparation industry. This next-generation sequencing library preparation market research report delivers a complete perspective of everything you need, with an in-depth analysis of the current and future scenario of the industry.
Next-generation sequencing (NGS) library preparation refers to the process of preparing DNA or RNA samples for sequencing. This involves amplifying the genetic material and adding specific tags so that sequencing machines can accurately read and interpret the data. Proper library preparation is essential for ensuring high-quality and reliable results, enabling detailed studies of genes, diseases, and other biological processes.
The priamry types of NGS library preparation include targeted genome sequencing, whole genome sequencing, whole exome sequencing, and others. Targeted genome sequencing is especially valued for its high-depth coverage and cost-efficient detection of mutations in specific genomic regions, making it suitable for both research and diagnostic applications. Key products include reagents and consumables, DNA library preparation kits, RNA library preparation kits, and other related materials. The technologies supporting these preparations include ion semiconductor sequencing, sequencing by synthesis (SBS), nanopore sequencing, single-molecule real-time (SMRT) sequencing, and others. NGS library preparation finds applications in drug and biomarker discovery, disease diagnostics, cancer diagnostics, reproductive health diagnostics, infectious disease diagnostics, and more. End users include hospitals and clinics, academic and research institutions, pharmaceutical and biotechnology companies, among others.
The next-generation sequencing library preparation market consists of revenues earned by entities by providing services such as sample preparation and extraction, quantification and quality assessment, amplification and enrichment, quality control and validation, and barcode tagging. The market value includes the value of related goods sold by the service provider or included within the service offering. The next-generation sequencing (NGS) library preparation market also includes sales of automated liquid handling systems, thermal cyclers, fragmentation systems, and barcoding and sample tracking systems. Values in this market are ‘factory gate’ values, that is, the value of goods sold by the manufacturers or creators of the goods, whether to other entities (including downstream manufacturers, wholesalers, distributors, and retailers) or directly to end customers. The value of goods in this market includes related services sold by their creators.
The market value is defined as the revenues that enterprises gain from the sale of goods and/or services within the specified market and geography through sales, grants, or donations in terms of the currency (in USD unless otherwise specified).
The revenues for a specified geography are consumption values that are revenues generated by organizations in the specified geography within the market, irrespective of where they are produced. It does not include revenues from resales along the supply chain, either further along the supply chain or as part of other products.
This product will be delivered within 1-3 business days.
The next-generation sequencing library preparation market size is expected to see rapid growth in the next few years. It will grow to $3.17 billion in 2030 at a compound annual growth rate (CAGR) of 14.2%. The growth in the forecast period can be attributed to development of automated library preparation platforms, growth of whole genome and exome sequencing, rising investment in personalized medicine, expansion of academic and research genomics projects, integration of ngs data with ai and cloud analytics. Major trends in the forecast period include rising demand for high-throughput sequencing technologies, expansion of ngs library preparation kits and reagents, increased focus on personalized medicine and biomarker discovery, growth in infectious disease and cancer diagnostics, adoption of automation in library preparation workflows.
The increasing adoption of gene therapy is expected to drive the growth of the next-generation sequencing (NGS) library preparation market in the coming years. Gene therapy involves modifying a person’s genes to treat or prevent diseases and is expanding due to the rising prevalence of genetic disorders that require targeted and durable treatment solutions. NGS library preparation supports gene therapy by enabling accurate analysis and validation of genetic modifications through the preparation of high-quality DNA or RNA samples for sequencing. For example, in March 2024, IQVIA, a U.S.-based healthcare intelligence and life sciences company, reported that in 2023, global spending on cell and gene therapies reached $5.9 billion, a 38% increase from 2022, even though it accounted for only 0.4% of the $1.6 trillion total global medicines expenditure. Consequently, the rise of gene therapy is fueling the growth of the NGS library preparation market.
Companies in the NGS library preparation market are focusing on advanced technologies such as Xpandomer technology to enhance sequencing speed, improve data accuracy, and increase scalability, streamlining workflows for more efficient and reliable genomic analysis. Xpandomer technology converts a target DNA or RNA molecule into a synthetic expanded polymer called an Xpandomer, which is 50 times longer than the original strand. For instance, in February 2025, Roche AG, a Switzerland-based biotechnology company, launched Sequencing by Expansion, a next-generation sequencing platform designed to improve genomic resolution for advanced diagnostics, disease research, and precision medicine. This automated NGS library preparation system enhances data quality, accelerates genomic analysis, and allows precise detection of genomic alterations across a wide range of diseases, including cancer and rare genetic disorders.
In January 2023, Agilent Technologies Inc., a US-based biotechnology company, acquired Avida Biomed for an undisclosed amount. This acquisition strengthens Agilent’s capabilities in cancer research and diagnostics by integrating advanced NGS tools with its existing platforms, enhancing precision medicine applications such as biomarker discovery and therapy selection. Avida Biomed is a US-based biotechnology firm specializing in high-performance NGS target enrichment workflows for cancer research.
Major companies operating in the next-generation sequencing library preparation market are F. Hoffmann-La Roche Ltd., Thermo Fisher Scientific Inc., Agilent Technologies India Private Limited, Illumina Inc., Beckman Coulter Inc., Bio-Rad Laboratories Inc., IDT Corporation, Tecan Group AG, Hamilton Company, Takara Bio Inc., 10x Genomics Inc., Oxford Nanopore Technologies Plc, Twist Bioscience Corporation, New England Biolabs Inc., SPT Labtech Limited, Watchmaker Genomics Inc., Pillar Biosciences Inc., Aurora Biomed Inc., BGI Group, SEQWELL INC.
North America was the largest region in the next-generation sequencing library preparation market in 2025. Asia-Pacific is expected to be the fastest-growing region in the forecast period. The regions covered in the next-generation sequencing library preparation market report are Asia-Pacific, South East Asia, Western Europe, Eastern Europe, North America, South America, Middle East, Africa. The countries covered in the next-generation sequencing library preparation market report are Australia, Brazil, China, France, Germany, India, Indonesia, Japan, Taiwan, Russia, South Korea, UK, USA, Canada, Italy, Spain.
Tariffs are impacting the next-generation sequencing library preparation market by increasing the cost of imported reagents, consumables, and sequencing kits. Segments such as DNA and RNA library preparation kits are particularly affected, with Asia-Pacific regions including China and India facing high import duties. This has led to higher production costs and potential delays in supply. On the positive side, tariffs are encouraging local manufacturing, development of alternative reagents, and investment in domestic supply chains, which may strengthen regional capabilities over time.
The next-generation sequencing library preparation market research report is one of a series of new reports that provides next-generation sequencing library preparation market statistics, including next-generation sequencing library preparation industry global market size, regional shares, competitors with a next-generation sequencing library preparation market share, detailed next-generation sequencing library preparation market segments, market trends and opportunities, and any further data you may need to thrive in the next-generation sequencing library preparation industry. This next-generation sequencing library preparation market research report delivers a complete perspective of everything you need, with an in-depth analysis of the current and future scenario of the industry.
Next-generation sequencing (NGS) library preparation refers to the process of preparing DNA or RNA samples for sequencing. This involves amplifying the genetic material and adding specific tags so that sequencing machines can accurately read and interpret the data. Proper library preparation is essential for ensuring high-quality and reliable results, enabling detailed studies of genes, diseases, and other biological processes.
The priamry types of NGS library preparation include targeted genome sequencing, whole genome sequencing, whole exome sequencing, and others. Targeted genome sequencing is especially valued for its high-depth coverage and cost-efficient detection of mutations in specific genomic regions, making it suitable for both research and diagnostic applications. Key products include reagents and consumables, DNA library preparation kits, RNA library preparation kits, and other related materials. The technologies supporting these preparations include ion semiconductor sequencing, sequencing by synthesis (SBS), nanopore sequencing, single-molecule real-time (SMRT) sequencing, and others. NGS library preparation finds applications in drug and biomarker discovery, disease diagnostics, cancer diagnostics, reproductive health diagnostics, infectious disease diagnostics, and more. End users include hospitals and clinics, academic and research institutions, pharmaceutical and biotechnology companies, among others.
The next-generation sequencing library preparation market consists of revenues earned by entities by providing services such as sample preparation and extraction, quantification and quality assessment, amplification and enrichment, quality control and validation, and barcode tagging. The market value includes the value of related goods sold by the service provider or included within the service offering. The next-generation sequencing (NGS) library preparation market also includes sales of automated liquid handling systems, thermal cyclers, fragmentation systems, and barcoding and sample tracking systems. Values in this market are ‘factory gate’ values, that is, the value of goods sold by the manufacturers or creators of the goods, whether to other entities (including downstream manufacturers, wholesalers, distributors, and retailers) or directly to end customers. The value of goods in this market includes related services sold by their creators.
The market value is defined as the revenues that enterprises gain from the sale of goods and/or services within the specified market and geography through sales, grants, or donations in terms of the currency (in USD unless otherwise specified).
The revenues for a specified geography are consumption values that are revenues generated by organizations in the specified geography within the market, irrespective of where they are produced. It does not include revenues from resales along the supply chain, either further along the supply chain or as part of other products.
This product will be delivered within 1-3 business days.
Table of Contents
1. Executive Summary
2. Next-Generation Sequencing Library Preparation Market Characteristics
3. Next-Generation Sequencing Library Preparation Market Supply Chain Analysis
4. Global Next-Generation Sequencing Library Preparation Market Trends and Strategies
5. Next-Generation Sequencing Library Preparation Market Analysis of End Use Industries
7. Global Next-Generation Sequencing Library Preparation Strategic Analysis Framework, Current Market Size, Market Comparisons and Growth Rate Analysis
8. Global Next-Generation Sequencing Library Preparation Total Addressable Market (TAM) Analysis for the Market
9. Next-Generation Sequencing Library Preparation Market Segmentation
10. Next-Generation Sequencing Library Preparation Market Regional and Country Analysis
11. Asia-Pacific Next-Generation Sequencing Library Preparation Market
12. China Next-Generation Sequencing Library Preparation Market
13. India Next-Generation Sequencing Library Preparation Market
14. Japan Next-Generation Sequencing Library Preparation Market
15. Australia Next-Generation Sequencing Library Preparation Market
16. Indonesia Next-Generation Sequencing Library Preparation Market
17. South Korea Next-Generation Sequencing Library Preparation Market
18. Taiwan Next-Generation Sequencing Library Preparation Market
19. South East Asia Next-Generation Sequencing Library Preparation Market
20. Western Europe Next-Generation Sequencing Library Preparation Market
21. UK Next-Generation Sequencing Library Preparation Market
22. Germany Next-Generation Sequencing Library Preparation Market
23. France Next-Generation Sequencing Library Preparation Market
24. Italy Next-Generation Sequencing Library Preparation Market
25. Spain Next-Generation Sequencing Library Preparation Market
26. Eastern Europe Next-Generation Sequencing Library Preparation Market
27. Russia Next-Generation Sequencing Library Preparation Market
28. North America Next-Generation Sequencing Library Preparation Market
29. USA Next-Generation Sequencing Library Preparation Market
30. Canada Next-Generation Sequencing Library Preparation Market
31. South America Next-Generation Sequencing Library Preparation Market
32. Brazil Next-Generation Sequencing Library Preparation Market
33. Middle East Next-Generation Sequencing Library Preparation Market
34. Africa Next-Generation Sequencing Library Preparation Market
36. Next-Generation Sequencing Library Preparation Market Competitive Landscape and Company Profiles
37. Next-Generation Sequencing Library Preparation Market Other Major and Innovative Companies
40. Next-Generation Sequencing Library Preparation Market High Potential Countries, Segments and Strategies
41. Appendix
List of Tables
Executive Summary
Next-Generation Sequencing Library Preparation Market Global Report 2026 provides strategists, marketers and senior management with the critical information they need to assess the market.This report focuses next-generation sequencing library preparation market which is experiencing strong growth. The report gives a guide to the trends which will be shaping the market over the next ten years and beyond.
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Description
Where is the largest and fastest growing market for next-generation sequencing library preparation? How does the market relate to the overall economy, demography and other similar markets? What forces will shape the market going forward, including technological disruption, regulatory shifts, and changing consumer preferences? The next-generation sequencing library preparation market global report answers all these questions and many more.The report covers market characteristics, size and growth, segmentation, regional and country breakdowns, total addressable market (TAM), market attractiveness score (MAS), competitive landscape, market shares, company scoring matrix, trends and strategies for this market. It traces the market’s historic and forecast market growth by geography.
- The market characteristics section of the report defines and explains the market. This section also examines key products and services offered in the market, evaluates brand-level differentiation, compares product features, and highlights major innovation and product development trends.
- The supply chain analysis section provides an overview of the entire value chain, including key raw materials, resources, and supplier analysis. It also provides a list competitor at each level of the supply chain.
- The updated trends and strategies section analyses the shape of the market as it evolves and highlights emerging technology trends such as digital transformation, automation, sustainability initiatives, and AI-driven innovation. It suggests how companies can leverage these advancements to strengthen their market position and achieve competitive differentiation.
- The regulatory and investment landscape section provides an overview of the key regulatory frameworks, regularity bodies, associations, and government policies influencing the market. It also examines major investment flows, incentives, and funding trends shaping industry growth and innovation.
- The market size section gives the market size ($b) covering both the historic growth of the market, and forecasting its development.
- The forecasts are made after considering the major factors currently impacting the market. These include the technological advancements such as AI and automation, Russia-Ukraine war, trade tariffs (government-imposed import/export duties), elevated inflation and interest rates.
- The total addressable market (TAM) analysis section defines and estimates the market potential compares it with the current market size, and provides strategic insights and growth opportunities based on this evaluation.
- The market attractiveness scoring section evaluates the market based on a quantitative scoring framework that considers growth potential, competitive dynamics, strategic fit, and risk profile. It also provides interpretive insights and strategic implications for decision-makers.
- Market segmentations break down the market into sub markets.
- The regional and country breakdowns section gives an analysis of the market in each geography and the size of the market by geography and compares their historic and forecast growth.
- Expanded geographical coverage includes Taiwan and Southeast Asia, reflecting recent supply chain realignments and manufacturing shifts in the region. This section analyzes how these markets are becoming increasingly important hubs in the global value chain.
- The competitive landscape chapter gives a description of the competitive nature of the market, market shares, and a description of the leading companies. Key financial deals which have shaped the market in recent years are identified.
- The company scoring matrix section evaluates and ranks leading companies based on a multi-parameter framework that includes market share or revenues, product innovation, and brand recognition.
Report Scope
Markets Covered:
1) By Sequencing Type: Targeted Genome Sequencing; Whole Genome Sequencing; Whole Exome Sequencing; Other Sequencing Types2) By Product: Reagents And Consumables; DNA Library Preparation Kits; Library Preparation Kits; RNA Library Preparation Kits; Other Products
3) By Technology: Ion Semiconductor Sequencing; Sequencing By Synthesis (SBS); Nanopore Sequencing; Single-Molecule Real-Time Sequencing (SMRT); Other Technologies
4) By Application: Drug And Biomarker Discovery; Disease Diagnostics; Cancer Diagnostics; Reproductive Health Diagnostics; Infectious Disease Diagnostics; Other Applications
5) By End-User: Hospitals And Clinics; Academic And Research Institutions; Pharmaceutical And Biotechnology Companies; Other End Users
Subsegments:
1) By Targeted Genome Sequencing: Amplicon Sequencing; Hybridization Capture-Based Sequencing; CRISPR-Based Target Enrichment; Multiplex PCR2) By Whole Genome Sequencing: Human Whole Genome Sequencing; Animal Whole Genome Sequencing; Plant Whole Genome Sequencing; Microbial Whole Genome Sequencing
3) By Whole Exome Sequencing: Clinical Whole Exome Sequencing; Research Whole Exome Sequencing; Custom Exome Sequencing; Population-Scale Exome Sequencing
4) By Other Sequencing Types: RNA Sequencing; ChIP Sequencing (ChIP-Seq); Methylation Sequencing; Single-Cell Sequencing
Companies Mentioned: F. Hoffmann-La Roche Ltd.; Thermo Fisher Scientific Inc.; Agilent Technologies India Private Limited; Illumina Inc.; Beckman Coulter Inc.; Bio-Rad Laboratories Inc.; IDT Corporation; Tecan Group AG; Hamilton Company; Takara Bio Inc.; 10x Genomics Inc.; Oxford Nanopore Technologies Plc; Twist Bioscience Corporation; New England Biolabs Inc.; SPT Labtech Limited; Watchmaker Genomics Inc.; Pillar Biosciences Inc.; Aurora Biomed Inc.; BGI Group; SEQWELL INC.
Countries: Australia; Brazil; China; France; Germany; India; Indonesia; Japan; Taiwan; Russia; South Korea; UK; USA; Canada; Italy; Spain
Regions: Asia-Pacific; South East Asia; Western Europe; Eastern Europe; North America; South America; Middle East; Africa
Time Series: Five years historic and ten years forecast.
Data: Ratios of market size and growth to related markets, GDP proportions, expenditure per capita.
Data Segmentation: Country and regional historic and forecast data, market share of competitors, market segments.
Sourcing and Referencing: Data and analysis throughout the report is sourced using end notes.
Delivery Format: Word, PDF or Interactive Report + Excel Dashboard
Added Benefits:
- Bi-Annual Data Update
- Customisation
- Expert Consultant Support
Companies Mentioned
The companies featured in this Next-Generation Sequencing Library Preparation market report include:- F. Hoffmann-La Roche Ltd.
- Thermo Fisher Scientific Inc.
- Agilent Technologies India Private Limited
- Illumina Inc.
- Beckman Coulter Inc.
- Bio-Rad Laboratories Inc.
- IDT Corporation
- Tecan Group AG
- Hamilton Company
- Takara Bio Inc.
- 10x Genomics Inc.
- Oxford Nanopore Technologies Plc
- Twist Bioscience Corporation
- New England Biolabs Inc.
- SPT Labtech Limited
- Watchmaker Genomics Inc.
- Pillar Biosciences Inc.
- Aurora Biomed Inc.
- BGI Group
- SEQWELL INC.
Table Information
| Report Attribute | Details |
|---|---|
| No. of Pages | 250 |
| Published | February 2026 |
| Forecast Period | 2026 - 2030 |
| Estimated Market Value ( USD | $ 1.86 Billion |
| Forecasted Market Value ( USD | $ 3.17 Billion |
| Compound Annual Growth Rate | 14.2% |
| Regions Covered | Global |
| No. of Companies Mentioned | 21 |
