Methods in Systems Bioinorganic Chemistry, Volume 741 provides understanding of high-level properties involving metals in biology that are founded on molecular or mechanistic level interactions. Currently, there is no established “tool kit” for studying bioinorganic systems, so the methods included in the book span a diverse range of topics and approaches, from imaging to proteomics, from metals-in-mice to mathematical modeling. Specific chapters cover Inductively Coupled Plasma Mass Spectrometry (ICP-MS)-based methods to quantify transition metals in cells and vertebrate models, ICP-MS elemental analysis in tissue samples, and much more.
Additional chapters delve into An inductively-coupled-plasma triple quadrupole method with oxygen for the determination of essential elements and protein concentration, Calculating metal speciation of proteins inside cells, Probing biological copper in the subfemtomolar regime, Using LC-ICP-MS chromatography to detect and characterize Labile Metal Pools, Linking dynamic bioinorganic chemistry processes of toxic metals in the bloodstream to establish their exposure-response relationship in humans, Contextualizing X-ray fluorescent microscopy - high resolution X-rays meet subcellular morphology, Chemoproteomic profiling of transition metal binding sites in bacterial proteomes, Heme Labeling by Proximity (HeLP) to Identify Hemoproteins, and much more.
Additional chapters delve into An inductively-coupled-plasma triple quadrupole method with oxygen for the determination of essential elements and protein concentration, Calculating metal speciation of proteins inside cells, Probing biological copper in the subfemtomolar regime, Using LC-ICP-MS chromatography to detect and characterize Labile Metal Pools, Linking dynamic bioinorganic chemistry processes of toxic metals in the bloodstream to establish their exposure-response relationship in humans, Contextualizing X-ray fluorescent microscopy - high resolution X-rays meet subcellular morphology, Chemoproteomic profiling of transition metal binding sites in bacterial proteomes, Heme Labeling by Proximity (HeLP) to Identify Hemoproteins, and much more.
Table of Contents
1. Inductively Coupled Plasma Mass Spectrometry (ICP-MS)-based methods to quantify transition metals in cells and vertebrate modelsVishal Gohil, Natalie M. Garza and Fotoula T. Stroumpos
2. ICP-MS elemental analysis in tissue samples
Oleh Khalimonchuk and Javier Seravalli
3. An inductively-coupled-plasma triple quadrupole method with oxygen for the determination of essential elements and protein concentration
Anne Roberts and Blaine Roberts
4. Calculating metal speciation of proteins inside cells
Nigel J. Robinson and Arthur Glasfeld
5. Probing biological copper in the subfemtomolar regime
Christoph J. Fahrni
6. Using LC-ICP-MS chromatography to detect and characterize Labile Metal Pools
Paul Lindahl and Alexia C. Kreinbrink
7. Linking dynamic bioinorganic chemistry processes of toxic metals in the bloodstream to establish their exposure-response relationship in humans
J�rgen Gailer and Yemna Badar
8. Contextualizing X-ray fluorescent microscopy high resolution X-rays meet subcellular morphology
Martina Ralle
9. Chemoproteomic profiling of transition metal binding sites in bacterial proteomes
David P. Giedroc, Maximillian K. Osterberg, Daniel W. Bak and Eranthie Weerapana
10. Heme Labeling by Proximity (HeLP) to Identify Hemoproteins
Amit R. Reddi
11. Profiling of metalloproteomes by METAL-TPP
Chu Wang
12. Using omics to understand the disorders of copper misbalance
Lorena Molina and Svetlana Lutsenko
13. Interrogating iron and manganese absorption, distribution, and excretion in mice using radioisotopes
Thomas Bartnikas, Milan Prajapati and Mitchell Knutson
14. Making sense of mammalian iron physiology through computational modeling
Pedro Mendes
15. Integrating Mechanistic and Data-Driven Approaches to Modeling Iron Transport in Epithelial Cells
Cristian Salgado
16. The Basic Pathways Approach to designing dynamical mathematical models of metal metabolism in growing cells
Paul Lindahl, Jay R. Walton and Justin Sun
