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Exonucleases are enzymes that catalyze the hydrolysis of single-stranded nucleic acids, such as DNA and RNA. They are involved in a variety of biological processes, including DNA replication, repair, and recombination. Exonucleases are also used in the laboratory for a variety of applications, such as DNA sequencing, cloning, and PCR.
Exonucleases are divided into two main classes: 3' to 5' exonucleases and 5' to 3' exonucleases. 3' to 5' exonucleases cleave nucleic acids from the 3' end to the 5' end, while 5' to 3' exonucleases cleave nucleic acids from the 5' end to the 3' end. Exonucleases are also classified according to their substrate specificity, with some being specific to either DNA or RNA, while others are able to cleave both.
Exonucleases are widely used in the biotechnology and pharmaceutical industries, as well as in academic and clinical research. They are used in a variety of applications, such as DNA sequencing, cloning, and PCR.
Some companies in the exonuclease market include New England Biolabs, Promega, Thermo Fisher Scientific, Takara Bio, and Agilent Technologies. Show Less Read more